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Oriented purple-membrane films as a probe for studies of the mechanism of bacteriorhodopsin functioning. II. Photoelectric processesстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 31 декабря 2013 г.
- Авторы: Kononenko Alexander A., Lukashev Evgenii P., Chamorovsky Sergei K., Maximychev Alexander V., Timashev Sergei F., Chekulaeva Lina N., Rubin Andrew B., Paschenko Vladimir Z.
- Журнал: Biochimica et Biophysica Acta - Bioenergetics
- Том: 892
- Номер: 1
- Год издания: 1987
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 56
- Последняя страница: 67
- DOI: 10.1016/0005-2728(87)90247-7
- Аннотация: Photoelectric processes have been investigated in dry orderly oriented preparations of purple membranes from Halobacterium halobium under both continuous light and flash excitation. An electrophoretic sedimentation on Al, Cu, Fe, Ni, Pt and SnO2 substrates was used to obtain orderly oriented purple-membrane films. The photoelectric response of the purple-membrane film is the sum of a light-induced ‘displacement’ current and a constant steady-state current, the proportion between the two depending upon the chemical nature of the electrodes and humidity of the film. With a high humidity, the steady-state photocurrent is correlated with the reactivity of the cathode metal (toward H+ reduction reaction. A correlation is found to exist between the kinetics of photopotential rise and decay and formation and decay of the K630 and M412 intermediates of the bacteriorhodopsin photocycle at temperatures ranging from 293 to 83 K, indicating the electrogenic nature of these intermediates. In purple-membrane films deposited on an SnO2 substrate, a correlation exists between the ‘dark’ potential and electron work function of the second electrode.
- Добавил в систему: Пащенко Владимир Захарович