NKCC-1 mediated Cl− uptake in immature CA3 pyramidal neurons is sufficient to compensate phasic GABAergic inputsстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 17 февраля 2021 г.
Аннотация:Activation of GABAA receptors causes in immature neurons a functionally relevant decrease in the intracellular Cl- concentration ([Cl-]i), a process termed ionic plasticity. Amount and duration of ionic plasticity depends on kinetic properties of [Cl-]i homeostasis. In order to characterize the capacity of Cl- accumulation and to quantify the effect of persistent GABAergic activity on [Cl-]i, we performed gramicidin-perforated patch-clamp recordings from CA3 pyramidal neurons of immature (postnatal day 4-7) rat hippocampal slices. These experiments revealed that inhibition of NKCC1 decreased [Cl-]i toward passive distribution with a time constant of 381 s. In contrast, active Cl- accumulation occurred with a time constant of 155 s, corresponding to a rate of 15.4 µM/s. Inhibition of phasic GABAergic activity had no significant effect on steady state [Cl-]i. Inhibition of tonic GABAergic currents induced a significant [Cl-]i increase by 1.6 mM, while activation of tonic extrasynaptic GABAA receptors with THIP significantly reduced [Cl-]i.. Simulations of neuronal [Cl-]i homeostasis supported the observation, that basal levels of synaptic GABAergic activation do not affect [Cl-]i. In summary, these results indicate that active Cl--uptake in immature hippocampal neurons is sufficient to maintain stable [Cl-]i at basal levels of phasic and to some extent also to compensate tonic GABAergic activity.