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Effect of albumin on the transformation of dinitrosyl iron complexes with thiourea ligandsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 ноября 2020 г.
- Авторы: Pokidova Оlesya V., Luzhkov Victor B., Emel'yanova Nina S., Krapivin Vladimir B., Kotelnikov Alexander I., Sanina Natalia A., Aldoshin Sergey M.
- Журнал: Dalton Transactions
- Том: 49
- Номер: 36
- Год издания: 2020
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 12674
- Последняя страница: 12685
- DOI: 10.1039/d0dt02452j
- Аннотация: Interaction and transformation of the mononuclear cationic dinitrosyl iron complex (Fe(SC(NH2)(2))(2)(NO)(2)](+) (complex 1) upon binding with bovine serum albumin (BSA) have been explored using kinetic measurements, UV-Vis and fluorescence spectroscopy, and computational molecular modeling. BSA was found to bind up to five molecules of complex 1 per one protein molecule; as a result, the rate of NO release by complex 1 into solution decreases by a factor of 10. The binding constant of complex 1 with BSA measured by the quenching of intrinsic fluorescence of BSA is 5 x 10(5) M-1. Molecular docking calculations at pH = 7 have determined five-six low-energy binding sites for complex 1 at subunits I and II of BSA. The most stable protein-ligand complexes are located at the protein pockets near Cys34. The spectroscopic measurements and docking calculations have shown that the decomposition product of complex 1, the Fe(NO)(2+) fragment, can form an adduct Fe(Cys34)(His39)(NO)(2) (complex 2) with the coordination bonds of Fe with atoms S of Cys34 and ND of His39. The structure of complex 2 was supported by the density functional calculations of the absorption spectrum. Decomposition of complex 2 leads to nitrosylation of BSA at atom S of Cys34. Complexes 1 (bound with BSA), 2 and the nitrosylated BSA can serve as NO depot in plasma.
- Добавил в систему: Алдошин Сергей Михайлович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Effect_of_albumin-Pokidova.pdf | 2,7 МБ | 12 декабря 2020 [kotel] |