|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Testing of geroprotectors in experiments on cell cultures: pros and consстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 1 апреля 2017 г.
- Авторы: Khokhlov A.N., Morgunova G.V.
- Сборник: Anti-aging Drugs: From Basic Research to Clinical Practice
- Серия: RSC Drug Discovery
- Глава в коллективной монографии
- Год издания: 2017
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 53
- Последняя страница: 74
- DOI: 10.1039/9781782626602-00051
- Аннотация: The paper is a critical review of various approaches to testing of geroprotectors (anti-aging compounds or physical factors) in experiments on cultured cells. The most popular cytogerontological models are reviewed with a focus on general gerontological definitions (aging, cell senescence, biomarkers of aging, aging and non-aging organisms, geroprotectors, age-related diseases, etc.). Among the model systems – the Hayflick model, the stationary phase aging model, the cell kinetics model. The main attention has paid to methodological aspects of the testing of geroprotectors with the models. Questions arising when constructing survival curves for cultured cells in the "stationary phase aging" model are analyzed. In particular, consideration is given to some problems encountered when using the most widespread molecular probes designed for live/dead cell viability assays. A note is made that the evaluation of colony-forming efficiency, though optimal for cell viability assessment, is unfortunately not applicable to postmitotic or very slowly propagating cells. Some questions regarding the interpretation of data obtained in such studies in application to humans are also considered. Popular approaches to choosing biomarkers of cell aging/senescence are briefly reviewed. It is assumed that the least number of problems associated with interpreting the results of testing potential geroprotectors in cytogerontological experiments arises when such studies are performed using the model of stationary phase aging (which is based on the concept of cell proliferation restriction as the main cause of accumulation of macromolecular lesions in cells of multicellular organisms with age) of normal cells. However, in the authors' opinion, even this approach will not give the final answer to the question of whether or not the studied factor is a geroprotector. Answering this question will inevitably require both "classic" gerontological experiments in animals and clinical trials.
- Добавил в систему: Моргунова Галина Васильевна