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Fetal mitochondrial DNA in maternal plasma in surrogate pregnancies: Detection and topologyстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 30 декабря 2020 г.
- Авторы: Ma Mary‐Jane L., Yakovenko Sergey, Zhang Haiqiang, Cheng Suk Hang, Apryshko Valentina, Zhavoronkov Alex, Jiang Peiyong, Chan K.C.Allen, Chiu Rossa W.K., Lo Y M.Dennis
- Журнал: Prenatal Diagnosis
- Год издания: 2020
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 1
- Последняя страница: 8
- Номер статьи: pd.5860
- DOI: 10.1002/pd.5860
- Аннотация: AbstractObjectives: Due to the maternally-inherited nature of mitochondrial DNA (mtDNA),there is a lack of information regarding fetal mtDNA in the plasma of pregnantwomen. We aim to explore the presence and topologic forms of circulating fetal andmaternal mtDNA molecules in surrogate pregnancies.Methods: Genotypic differences between fetal and surrogate maternal mtDNA wereused to identify the fetal and maternal mtDNA molecules in plasma. Plasma sampleswere obtained from the surrogate pregnant mothers. Using cleavage-end signaturesof BfaI restriction enzyme, linear and circular mtDNA molecules in maternal plasmacould be differentiated.Results: Fetal-derived mtDNA molecules were mainly linear (median: 88%; range:80%–96%), whereas approximately half of the maternal-derived mtDNA moleculeswere circular (median: 51%; range: 42%–60%). The fetal DNA fraction of linearmtDNA was lower (median absolute difference: 9.8%; range: 1.1%–27%) than that ofnuclear DNA (median: 20%; range: 9.7%–35%). The fetal-derived linear mtDNA molecules were shorter than the maternal-derived ones.Conclusion: Fetal mtDNA is present in maternal plasma, and consists mainly of linearmolecules. Surrogate pregnancies represent a valuable clinical scenario for exploringthe biology and potential clinical applications of circulating mtDNA, for example, forpregnancies conceived following mitochondrial replacement therapy.
- Добавил в систему: Яковенко Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | статья в pdf | Fetal_mitochondrial_DNA_in_maternal_plasma_in_surrogate.pdf | 2,1 МБ | 13 ноября 2020 [SAYakovenko] |