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Changes in cell wall structure and protein set in Candida maltosa grown on hexadecaneстатья
- Авторы: Zvonarev Anton, Farofonova Vasilina, Kulakovskaya Ekaterina, Kulakovskaya Tatiana, Machulin Andrey, Sokolov Svyatoslav, Dmitriev Vladimir
- Журнал: Folia Microbiologica
- Том: 1
- Номер: 27
- Год издания: 2020
- Издательство: Academy of Sciences of the Czech Republic
- Местоположение издательства: Czech Republic
- DOI: 10.1007/s12223-020-00840-2
- Аннотация: The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall whenconsuming water-insoluble nutrient sources. The cells of C. maltosa that utilize hydrocarbons contain supramolecular structures, so-called “canals” in the cell wall. Differences in protein profiles of culture liquids and cell wall extracts of C. maltosa grown on glucose and hexadecane were analyzed. Three proteins specific of cells grown on hexadecane were revealed using mass spectrometry: glycosyl hydrolase EPD2 in the culture liquid; a protein belonging to the cytochrome C family in the 0.5 mol/L NaCl extract; and PPIA_CANAL protein known as chaperone, in the 0.1% SDS extract. The possible role of these proteins in cell wall structures responsible for adaptation to hexadecane utilization is discussed.
- Добавил в систему: Соколов Святослав Сергеевич