HLDF-6-AMIDE REDUCES THE CYTOTOXICITY OF DOXORUBICIN AND ACTIVATES THE PROLIFERATION OF TETRAHYMENA PYRIFORMISстатья
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Дата последнего поиска статьи во внешних источниках: 29 апреля 2021 г.
Авторы:
Sokolov O.Yu ,
Pozdnyakova A.N. ,
Cheremnykh E.G. ,
Vasileva E.V.,
Kost N.V. ,
Zolotarev Y.A.
Журнал:
Russian Journal of Bioorganic Chemistry
Том:
46
Номер:
6
Год издания:
2020
Издательство:
Maik Nauka/Interperiodica Publishing
Местоположение издательства:
Russian Federation
Первая страница:
1034
Последняя страница:
1037
DOI:
10.1134/S1068162020060321
Аннотация:
ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2020, Vol. 46, No. 6, pp. 1034–1037. © Pleiades Publishing, Ltd., 2020.
Russian Text © The Author(s), 2020, published in Bioorganicheskaya Khimiya, 2020, Vol. 46, No. 6, pp. 666–669.
HLDF-6-Amide Reduces the Cytotoxicity of Doxorubicin and Activates the Proliferation of Tetrahymena Pyriformis
O. Yu. Sokolova, 1, A. N. Pozdnyakovaa, E. G. Cheremnykha, E. V. Vasilevac,
N. V. Kosta, and Y. A. Zolotarevb
a Mental Health Research Center, Russian Academy of Sciences, Moscow, 115552 Russia
b Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 119334 Russia c Zakusov Institute of Pharmacology, Moscow, 119334 Russia
Received April 9, 2020; revised April 16, 2020; accepted April 29, 2020
Abstract—How a number of biological effects of the peptide neuroprotector HLDF-6-amide work are still unknown and require further study. It was found that HLDF-6-amide dose-dependent (1–100 μM) stimulates the proliferation of Tetrahymena pyriformis infusoria, and the MK801 glutamate NMDA receptor antagonist partially blocks this effect. The introduction of a peptide (12.5–100 μM) reduces the cytotoxic and cytostatic effect of doxorubicin (25 μM) on infusoria. Radio-ligand analysis revealed the presence of specific binding sites of [3H] Ac-HLDF-6-amide on the cell membranes of the rat brain cortex, which indicates the receptor mechanism of action of the peptide. Thus, it was shown for the first time that HLDF-6-amide stimulates proliferation and has cytoprotective properties, which may underlie its wound-healing, nootropic and neuroprotective effects. The participation of the glutamate system, in particular ionotropic NMDA receptors, in these effects was established.
Keywords: peptide HLDF-6-amide, doxorubicin, MK 801, Tetrahymena pyriformis infusoria DOI: 10.1134/S1068162020060321
Добавил в систему:
Соколов Олег Юрьевич