Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIMстатья
Статья опубликована в высокорейтинговом журнале
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 февраля 2021 г.
Аннотация:Membrane fluidity plays an important role in many cell functions such as cell adhesion, and migration.In stem cell lines membrane fluidity may play a role in differentiation. Here we report the use ofviscosity-sensitive fluorophores based on a BODIPY core, termed “molecular rotors”, in combinationwith Fluorescence Lifetime Imaging Microscopy, for monitoring of plasma membrane viscositychanges in mesenchymal stem cells (MSCs) during osteogenic and chondrogenic differentiation.In order to correlate the viscosity values with membrane lipid composition, the detailed analysisof the corresponding membrane lipid composition of differentiated cells was performed by timeof-flight secondary ion mass spectrometry. Our results directly demonstrate for the first time thatdifferentiation of MSCs results in distinct membrane viscosities, that reflect the change in lipidome ofthe cells following differentiation.