|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Differences in the processes of accumulation, retention, and metabolism of the fluorescent dye rhodamine 123 in normal and tumor cellsтезисы доклада Тезисы
- Авторы: Zorova L.D., Demchenko E.A., Zorov S.D., Andrianova N.V., Popkov V.A., Babenko V.A., Danilina T.G., Pevzner I.B., Silachev D.N., Plotnikov E.Y., Zorov D.B.
- Сборник: Book of abstracts Sechenov International Biomedical Summit 2020
- Тезисы
- Год издания: 2020
- Место издания: Publishing house of Sechenov University, 2020, p. 52
- Первая страница: 45
- Последняя страница: 45
- Аннотация: Mitochondrial membrane potential (ΔΨm) is involved in a variety of cellular processes, justifying the importance of adequate ΔΨm estimation. The commonly used approach by the fluorescence intensity of dyes accumulated in mitochondria has a number of defects associated with different retention by normal and tumor cells. One possibility may be in the transformation of the original membrane-permeable into a non-permeable form. We analyzed butanol extracts obtained after incubation of astrocytes and glioma C6 cells with rhodamine 123 (R123) using TLC and mass-spectrometry and found a cell-type-dependent modifications of R123 (e.g., formation of deesterified R123 (R110) in both cell types). In glioma cells, components with molecular weight higher than of R123 were found. When assessing the dependence of the probe retention on ΔΨm after incubation of cells with R123 and uncoupler CCCP, differences were also observed: while fluorescence vanished in astrocytes after 5h, it still retained in glioma even after 24h. Incubation with R123 and amiodarone (inhibitor of P450 and non-specific pumps), R123 derivatives were not detected in glioma extracts, suggesting the participation of P450 in the metabolism of R123 in glioma. In astrocytes, an increase of total fluorescence was observed, indicating P450-dependent processing of R123, while glioma use other ways to modify the dye. Ultimately, covalent adducts and soluble impermeable fluorescent derivatives of R123 were found in glioma cells. No such modifications were found in astrocytes. Given the antitumor activity of R123, this data can be used in an anti-cancer strategy.
- Добавил в систему: Зорова Любава Дмитриевна