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Development of a Serum-Free Media Based on the Optimal Combination of Recombinant Protein Additives and Hydrolysates of Non-animal Origin to Produce Immunoglobulinsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 23 декабря 2020 г.
- Авторы: Balabashin D.S., Kaliberda E.N., Smirnov I.V., Mokrushina Y.A., Bobik T.V., Aliev T.K., Dolgikh D.A., Kirpichnikov M.P.
- Журнал: Applied Biochemistry and Microbiology
- Том: 56
- Номер: 5
- Год издания: 2020
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 595
- Последняя страница: 603
- DOI: 10.1134/s0003683820050038
- Аннотация: The potential ability to cultivate a recombinant immunoglobulin (IgG1)-producer line in Iscove's Modified Dulbecco Medium (IMDM) as the base cultivation medium with the introduction of recombinant proteins and hydrolysates of non-animal origin was studied. Recombinant insulin, human serum albumin (produced byE. colibacteria), and transferrin (byPichia pastorisyeasts) were used to enrich the medium. A combination of protein additives (part of the developed medium) allowed an increase in the productivity of a stable producer culture of recombinant humanized antibodies based on CHO-line cells by 44% as compared with the used complex commercial insulin-transferrin-selenium (ITS) additive. The introduction of pea and rice protein hydrolysates containing peptides with a molecular weight lower than 5 kDa to modified IMDM medium contributed to an increase in line productivity by 3.9 and 4.5 times, respectively.
- Добавил в систему: Бачева Анна Владимировна