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Comparative femtosecond spectroscopy of primary photoreactions of Exiguobacterium sibiricum rhodopsin and Halobacterium salinarum bacteriorhodopsinстатья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 апреля 2021 г.
- Авторы: Smitienko Olga, Feldman Tatiana, Petrovskaya Lada, Nekrasova Oksana, Yakovleva Marina, Shelaev Ivan, Gostev Fedor, Cherepanov Dmitry, Kolchugina Irina, Dolgikh Dmitry, Nadtochenko Victor, Kirpichnikov Mikhail, Ostrovsky Mikhail A.
- Журнал: Journal of Physical Chemistry B
- Том: 125
- Год издания: 2021
- Издательство: American Chemical Society
- Местоположение издательства: United States
- Первая страница: 995
- Последняя страница: 1008
- DOI: 10.1021/acs.jpcb.0c07763
- Аннотация: The primary stages of the Exiguobacterium sibiricum rhodopsin (ESR) photocycle were investigated using femtosecond absorption laser spectroscopy in the spectral range of 400900 nm with a time resolution of 25 fs. The dynamics of the ESR photoreaction were compared with the reactions of bacteriorhodopsin (bR) in purple membranes (bRPM) and in recombinant form (bRrec). The primary intermediates of the ESR photocycle were similar to intermediates I, J, and K in bacteriorhodopsin photoconversion. The CONTIN program was applied to analyze the characteristic times of the observed processes, and to clarify the reaction scheme. A similar photoreaction pattern was observed for all studied retinal proteins, including two consecutive dynamic Stokes shift phases lasting ~0.05 and ~0.15 ps. The excited state decays through a femtosecond reactive pathway, leading to retinal isomerization and formation of product J, and a picosecond non-reactive pathway that leads only to the initial state. Retinal photoisomerization in ESR takes 0.69 ps, compared with 0.48 ps in bRPM and 0.74 ps in bRrec. The non-reactive excited state decay takes 5 ps in ESR and ~3 ps in bR. We discuss the similarity of the primary reactions of ESR and other retinal proteins.
- Добавил в систему: Фельдман Татьяна Борисовна