|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
The action of extracellular calcium and the temperature of the isolation of lymphocytes on their viabilityстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 18 декабря 2013 г.
- Авторы: Kirillova G.P., Mikeshina N.G., Mokhova E.N., Turovetskii V.B., Volkov N.I.
- Журнал: Nauchnye doklady vysshe∎ shkoly. Biologicheskie nauki
- Номер: 3
- Год издания: 1990
- Издательство: Biologicheskie Nauki
- Местоположение издательства: Moskva, Russia (Federation)
- Первая страница: 20
- Последняя страница: 26
- Аннотация: The effect of extracellular calcium binding and temperature of isolation and storage of thymus lymphocytes on their viability was studied. The last was evaluated by the fluorescence intensity of fluoresceindiacetate stained cells and by the intracellular ATP content. The rise of temperature of isolation and storage of lymphocytes from 1 degree to 22 degrees C significantly lowered the intracellular ATP concentration in cells storing in dense suspension [(3-4) x 10(9) lymphocytes/ml]. However, the ATP content in lymphocytes increased as a result of short-term incubation by diluted suspension (3 x 10(7) lymphocytes/ml) at 22 degrees C. The fluorescence of fluorescein introduced into lymphocytes decrease in the presence of 1-5 mM EGTA (EDTA).
- Добавил в систему: Мохова Елена Николаевна