Cell wall polymers of Actinomadura carminata ИНА 4281статья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:The cell walls of Actinomadura carminata INA
4281 were found to contain peptidoglycan, teichoic acid,
and nonpeptidoglycan amino acids. The peptidoglycan was
of the A17 type and contained a small amount of LL-DAP
in addition to m-DAP. The teichoic acid was an 1,3-
poly(glycerol phosphate) chain composed of about eight
glycerophosphate units, two of which had a 2-acetamido-
2-deoxy-c~-D-galactopyranosyl substituent and one, a 3-0-
methyl-fl-D-galactopyranosyl-(1 --> 3)-2-acetamido-2-deoxy-
C~-D-galactopyranosyl residue at C2 of glycerol. The structure
of the polymer was identified by chemical analysis and 13C_
NMR spectroscopy. The teichoic acid contained 3-0-
methyl-D-galactose (madurose) - the first ever finding of
this compound within a teichoic acid. The nonpeptidoglycan
amino acids made up some 30% of the cell wall's dry weight,
about a quarter of the amino acids being removable with
sodium dodecyl sulfate. Further treatment of the cell walls
with LiC1 and guanidine hydrochloride caused only a small
loss of the amino acids and slight changes in their molar
ratio