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Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 июня 2021 г.
- Авторы: Fayzullin Alexey, Ignatieva Natalia, Zakharkina Olga, Tokarev Mark, Mudryak Daniil, Khristidis Yana, Balyasin Maxim, Kurkov Alexandr, Churbanov Semyon, Dyuzheva Tatyana, Timashev Peter, Guller Anna, Shekhter Anatoly
- Журнал: Biology
- Том: 10
- Номер: 2
- Год издания: 2021
- Издательство: MDPI Publishing
- Местоположение издательства: Basel, Switzerland, Switzerland
- Первая страница: 1
- Последняя страница: 15
- Номер статьи: 136
- DOI: 10.3390/biology10020136
- Аннотация: ature hypertrophic scars (HSs) remain a challenging clinical problem, particularly due to the absence of biologically relevant experimental models as a standard rabbit ear HS model only reflects an early stage of scarring. The current study aims to adapt this animal model for simulation of mature HS by validating the time of the scar stabilization using qualitative and quantitative criteria. The full-thickness skin and perichondrium excision wounds were created on the ventral side of the rabbit ears. The tissue samples were studied on post-operation days (PODs) 30, 60, 90 and 120. The histopathological examination and morphometry were applied in parallel with biochemical analysis of protein and glycosaminoglycans (GAGs) content and amino acid composition. The supramolecular organization of collagen was explored by differential scanning calorimetry. Four stages of the rabbit ear HS maturation were delineated and attributed with the histolomorphometrical and physicochemical parameters of the tissue. The experimental scars formed in 30 days but stabilized structurally and biochemically only on POD 90–120. This evidence-based model can be used for the studies and testing of new treatments of the mature HSs.
- Добавил в систему: Игнатьева Наталия Юрьевна