The Structure of the Potato Virus A Particles Elucidated by Small Angle X-Ray Scattering and Complementary Techniquesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 апреля 2021 г.
Аннотация:Potato virus A (PVA) protein coat contains on its surface partially unstructured N?terminal domain of the viral coat protein (CP), whose structural and functional characteristics are important for understanding the mechanism of plantinfection with this virus. In this work, we investigated the properties and the structure of intact PVA and partially trypsinizedPVAΔ32 virions using small angle X-ray scattering (SAXS) and complimentary methods. It was shown that after the removal of 32 N?terminal amino acids of the CP, the virion did not disintegrate and remained compact, but the helical pitch of theCP packing changed. To determine the nature of these changes, we performed ab initio modeling, including the multiphaseprocedure, with the geometric bodies (helices) and restoration of the PVA structure in solution using available high resolution structures of the homologous CP from the PVY potyvirus, based on the SAXS data. As a result, for the first time, a lowresolution structure of the filamentous PVA virus, both intact and partially degraded, was elucidated under conditions close to natural. The far UV circular dichroism spectra of the PVA and PVAΔ32 samples differed significantly in the amplitudeand position of the main negative maximum. The extent of thermal denaturation of these samples in the temperature range of 20-55°C was also different. The data of transmission electron microscopy showed that the PVAΔ32 virions were mostly rod-shaped, in contrast to the flexible filamentous particles typical of the intact virus, which correlated well with the SAXSresults. In general, structural analysis indicates an importance of the CP N?terminal domain for the vital functions of PVA, which can be used to develop a strategy for combating this plant pathogen.