Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA

Vdovenko, M.M.; Stepanova, A.S.; Eremin, S.A.; Nguyen, V.; Uskova, N.A.; Sakharov, I.Y.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 сентября 2013 г.

Авторы: Vdovenko Marina M., Stepanova Alexandra S., Eremin Sergei A., Nguyen Van Cuong, Uskova Natalia A., Sakharov Ivan Yu
Журнал: Food Chemistry
Том: 141
Год издания: 2013
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 865
Последняя страница: 868
DOI: 10.1016/j.foodchem.2013.04.060
Аннотация: Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4- dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4- D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimized. The chemiluminescent method based on peroxidase-catalyzed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'- phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545 ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4- D and containing different 2,4-D concentrations (10-300 ng/mL) were ranged from 92 to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of 5 oranges and 5 mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104 μg/kg) was significantly higher than that in mandarins (1.66- 2.82 μg/kg).
Добавил в систему: Еремин Сергей Александрович

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Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISAстатья

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