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Competitive and noncompetitive fluorescence polarization immunoassays for the detection of benzothiostrobin using FITC-labeled dendrimer-like peptidesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 августа 2021 г.
- Авторы: He Chen, You Tianyang, Zong Lingfeng, Mukhametova Liliya I., Zherdev Dmitry O., Eremin Sergei A., Ding Yuan, Wang Minghua, Hua Xiude
- Журнал: Food Chemistry
- Том: 360
- Год издания: 2021
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Номер статьи: 130020
- DOI: 10.1016/j.foodchem.2021.130020
- Аннотация: Peptides obtained from phage display libraries are valuable reagents for small-molecule immunoassays. However, their application in fluorescence polarization immunoassays (FPIAs) is limited by phage particles. Here, monomer, dendrimer-like dimer, tetramer peptidomimetic and anti-immunocomplex tracers were designed and synthesized using lysine as special scaffolds and spacers to develop competitive and noncompetitive FPIAs for benzothiostrobin. The affinity between tracers and monoclonal antibodies or immunocomplexes increased with the tracer valence. A higher signal-to-noise ratio and sensitivity could be generated in the FPIAs based on tetramer tracers. The sensitivities of competitive (50% inhibitory concentration) and noncompetitive (50% saturation concentration) FPIAs were 19.71 ± 4.65 and 40.43 ± 2.73 ng mL−1, respectively. The spiked recoveries were 78.3%−105.2% with relative standard deviations (RSDs) of 0.7%−15.4% for the competitive FPIA, while 78.7%−115.3% with RSDs of 0.7%−12.5% for the noncompetitive FPIA. The amounts of benzothiostrobin in rice detected by the FPIAs were consistent with those detected by high performance liquid chromatography.
- Добавил в систему: Еремин Сергей Александрович
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