hTERT-immortalized adipose-derived stem cell line ASC52Telo demonstrates limited potential for adipose biology research

Masnikov, D.; Stafeev, I.; Michurina, S.; Zubkova, E.; Mamontova, E.; Ratner, E.; Menshikov, M.; Parfyonova, Y.

Авторы: Masnikov D., Stafeev I., Michurina S., Zubkova E., Mamontova E., Ratner E., Menshikov M., Parfyonova Ye
Журнал: Analytical Biochemistry
Том: 628
Год издания: 2021
Издательство: Academic Press
Местоположение издательства: United States
Номер статьи: 114268
DOI: 10.1016/j.ab.2021.114268
Аннотация: In the modern world obesity and insulin resistance contribute to a high impact on the structure of mortality. Basic research and pharmacological screenings for the search of new targets and insulin sensitizers require relevant cell models of adipocytes. Today the 3T3-L1 preadipocytes cell line is a widely used mouse-based model for investigation of adipocyte biology. Nonetheless, animal studies cannot be transferred directly in human research and nowadays the search for relevant and renewable cell models of human adipocyte is of undeniable importance. In the present study, we have compared pooled culture of human adipose-derived stem cells (ADSC) with immortalized ADSC cell line ASC52Telo. Both cell types had mesenchymal stem cell phenotype verified by flow cytometry. However, the efficacy of adipogenic differentiation, stimulation of FABP4 and PPARg protein expressions, and glucose uptake stimulation by insulin were reduced for ASC52Telo-derived adipocytes in comparison with ADSC-derived adipocytes. In addition, the analysis of insulin signaling has shown impaired phosphorylation of IRS1 and AS160 in ASC52Telo-derived cells. In summary, we have shown that immortalized cell line of human ADSC ASC52Telo have mesenchymal stem cell phenotype. Nevertheless, ASC52Telo-derived adipocytes demonstrate impaired adipogenesis and insulin sensitivity that are the main properties of healthy adipocytes.
Добавил в систему: Стафеев Юрий Сергеевич

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hTERT-immortalized adipose-derived stem cell line ASC52Telo demonstrates limited potential for adipose biology researchстатья Электронная публикация

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