RNA-polymerase II-derived non-coding RNAs suppress mRNA export and abolish antiviral protection in plantsстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
Аннотация:RNA export from the nucleus to cytoplasm is an essential stage of gene expression. RNA species including mRNA, tRNA, rRNA and different small RNAs that are produced in the nucleus are exported through the nuclear pore complexes via mobile export receptors. To elucidate the role of RNA polymerases (Pol) in RNA export we exploited Agrobacterium tumefaciens ability to deliver into plant nucleus cDNA expressing (i) short non-coding RNAs (sncRNAs) under control of 35S (Pol II35S-sncRNAs) or tRNATyr (Pol IIItRNA-sncRNAs) promoter and (ii) GFP gene as a marker of mRNA export route. Using the technique based on MS2 bacteriophage coat protein ability to bind to MS2 19 base RNA we have shown that both types (Pol II35S- and Pol IIItRNA-) of RNA are exported to cytoplasm with high efficiency. In contrast to Pol IIItRNA-, all tested Pol II35S-sncRNAs (U6, tRNA, miRNAs or artificial RNA) drastically decreased GFP accumulation but insertion of intron into GFP gene partly restored GFP expression level.
We suggested that Pol II35S-sncRNAs moving into cytoplasm via mRNA export route may block nucleocytoplasmic mRNA traffic and impair functioning of main antiviral cell protection mechanism – RNA-silencing. To check this hypothesis we developed a model system which allowed us to control Tobacco mosaic virus or Potato virus X reproduction in plant cell. It has been shown that all tested Pol II35S-sncRNAs significantly increased viral RNA accumulation whereas Pol IIItRNA-sncRNA had no effect. Thus, we concluded that Pol II35S-sncRNA-mediated suppression of mRNA export may create the condition for uncontrollable virus reproduction.