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Four translation initiation pathways employed by the leaderless mRNA in eukaryotesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 января 2017 г.
- Авторы: Akulich Kseniya A., Andreev Dmitry E., Terenin Ilya M., Smirnova Victoria V., Anisimova Aleksandra S., Makeeva Desislava S., Arkhipova Valentina I., Stolboushkina Elena A., Garber Maria B., Prokofjeva Maria M., Spirin Pavel V., Prassolov Vladimir S., Shatsky Ivan N., Dmitriev Sergey E.
- Журнал: Scientific reports
- Том: 6
- Год издания: 2016
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 37905
- DOI: 10.1038/srep37905
- Аннотация: mRNAs lacking 5' untranslated regions (leaderless mRNAs) are molecular relics of an ancient translation initiation pathway. Nevertheless, they still represent a significant portion of transcriptome in some taxons, including a number of eukaryotic species. In bacteria and archaea, the leaderless mRNAs can bind non-dissociated 70 S ribosomes and initiate translation without protein initiation factors involved. Here we use the Fleeting mRNA Transfection technique (FLERT) to show that translation of a leaderless reporter mRNA is resistant to conditions when eIF2 and eIF4F, two key eukaryotic translation initiation factors, are inactivated in mammalian cells. We report an unconventional translation initiation pathway utilized by the leaderless mRNA in vitro, in addition to the previously described 80S-, eIF2-, or eIF2D-mediated modes. This mechanism is a bacterial-like eIF5B/IF2-assisted initiation that has only been reported for hepatitis C virus-like internal ribosome entry sites (IRESs). Therefore, the leaderless mRNA is able to take any of four different translation initiation pathways in eukaryotes.
- Добавил в систему: Дмитриев Сергей Евгеньевич