Evaluating the Applicability of alternative substrates in Hydrophilic interaction Chromatography

Popov, A.; Chernobrovkina, A.; Domnikov, K.; Smolenkov, A.; Shpigun, O.

Авторы: Popov Alexander, Chernobrovkina A., Domnikov K., Smolenkov A., Shpigun O.
Сборник: In book of Abstract 32d International Symposium on Chromatography
Тезисы
Год издания: 2018
Место издания: Cannes-Mandelieu
Первая страница: 76
Аннотация: Modern chromatographic methods using bonded phases based on silica are restricted in the choice of the eluent due to the limited substrate operating stability range (pH 2-8). The use of metal oxides or polymers that are stable in a wider pH range as alternative substrates can contribute not only an efficiency increase, but also an alternative separation selectivity due to the different electrostatic charge of their surface and the possibility of converting the analytes into appropriate ionic form, which is important for the separation of polar compounds in HILIC mode. In the present work alternative substrates for HILIC were explored, such as titanium dioxide (particle diameter of 5 μm) and polystyrenedivinylbenzene (particle diameter of 5.5 μm) with a crosslinking degree of 50% and a hydrophilic surface layer. Titanium dioxide has a wider spectrum of possible interactions with sorbates as compared with silica, namely, cationic, anionic, and also ligand-exchange ones. The retention characteristics for water-soluble vitamins (thiamine, nicotinic acid and amide, riboflavin, pyridoxine, cyanocobalamin) on the surface of titanium dioxide particles in HILIC mode were studied in the present work. Mobile phase parameters such as water/acetonitrile ratio, pH, type, and concentration of the buffer solution were varied to establish the optimal separation conditions. It was demonstrated that separation efficiency up to 20000 N/m could be achieved using 100mM ammonium acetate and pH 5.8, while selectivity increases with decreasing buffer concentration in the mobile phase down to 10-50 mM. Chemically and mechanically stable, but hydrophobic polystyrenedivinylbenzene (PS-DVB) was another tested substrate. The proposed novel method of its surface hydrophilization by covalent attachment of polyethyleneimine made it suitable for the separation of polar compounds in HILIC. The mixture of thiamine, riboflavin, cyanocobalamin, nicotinic, and ascorbic acids were separated in 20 min with efficiency of up to 10000 N/m in gradient elution mode using 100 mM ammonium acetate, pH 5.8 and 8-30% acetonitrile. The proposed substrates indicated new prospects in the development of HILIC method due to the increased chemical stability, expansion of the operating pH range, and also the alternative selectivity of separating polar compounds.
Добавил в систему: Попов Александр Сергеевич

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Evaluating the Applicability of alternative substrates in Hydrophilic interaction Chromatographyтезисы доклада

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