Recombinant Destabilase from the Medicinal leech: Preparation and Propertiesстатья
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Дата последнего поиска статьи во внешних источниках: 14 декабря 2016 г.
Аннотация:Abstract—The procedure for obtaining an active recombinant destabilase from the medicinal leech in
Escherichia coli cells was developed. The plasmids encoding an analogue of native destabilase, as well as the
protein forms carrying polyhistidine sequence at the C and/or Nterminus of the polypeptide were obtained
during the work. The producing strains of different forms of the protein were constructed, the cultivation pro
cess was optimized. The conditions of renaturation of destabilase recombinant forms by dialysis and using
chromatographic absorbent were selected. The muramidase activity towards cell walls of Micrococcus lyso
deikticus bacferia and lytic activity towards E. coli were investigated. The dependence of pH and ionic strength
of the solution on the activities was determined. The total antibacterial activity of destabilase towards E. coli
was shown.