Polymerization of (+)-Catechin in Deep Eutectic Solvent  with Fungal Laccase: Physicochemical Properties  of the Products and Inhibition of alpha-Glucosidase

Авторы: Khlupova M.E., Morozova O.V., Vasil’eva I.S., Shumakovich G.P., Zaitseva E.A., Chertkov V.A., Shestakova A.K., Yaropolov A.I.
Журнал: Applied Biochemistry and Microbiology
Том: 57
Номер: 6
Год издания: 2021
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 712
Последняя страница: 718
DOI: 10.1134/S0003683821060065
Аннотация: Deep eutectic solvents (DESs) are an alternative to traditional organic solvents for enzymatic reactions between compounds with poor solubility. Biocatalytic polymerization of the flavonoid (+)-catechin (CC)was carried out with laccase from the culture liquid of the basidiomycete Trametes hirsuta in a DES–buffer mixture (betaine/glycerol–buffer, 60 : 40 v/v). The conditions for the synthesis of catechin oligomers (oligoCCs)soluble in organic solvents were selected. According to the data from high-performance liquid chromatography,the oligoCCs had average molecular weights of 10 620 and 2540 g/mol with polydispersity indices of 1.1 and1.09, respectively. The physicochemical properties of the obtained oligomers were studied via UV-visible, FTIR,1H and 13C NMR spectroscopy. The resulting oligoCCs inhibited the α-glucosidase activity (IC50 ~ 8 μg/mL)
Добавил в систему: Чертков Вячеслав Алексеевич

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