Аннотация:The ability to produce secondary metabolites: inducible enzymes (some hydrolases and oxidases) and antibiotics for soil strains of micromycetes of the Arctic (Franz Josef Land, Novaya Zemlya) and Antarctica (oases of Tal Hill, Larsemann Hills, Schirmacher, Mary Bird Land) was evaluated. Maximum esterase activity was found in strains of typical Antarctic species Hyphozyma variabilis 218 and Thelebolus ellipsoideus 210 – 51 and 29 nmol PDA/g of mycelium per hour, respectively. The highest values of cellulolytic activity – 89 mol glucose/mg biomass – were observed in Ascochyta pisi 192. No activity of extracellular phenoloxidases laccases and peroxidases was found among the tested strains. Antibacterial activity against Bacillus subtilis ATCC 6633 was detected in 75% of the studied Antarctic micromycetes strains. Highly active strains were isolated from organic and moisture-rich biotopes with moss/lichen cover. The most active were Paecilomyces marquandii 166, Penicillium janczewskii 165, P. roseopurpureum 169 and Thelebolus ellipsoideus 210. Antagonistic activity against Antarctic bacterial strains was shown by 77% of the tested fungal strains. The maximum inhibition was found in strains typical of Antarctica Antarctomyces psychrotrophicus MT303855, as well as the eurytopic Sarocladium kiliense MT303856. Antimycotic activity was manifested in 42% of the studied strains. In 38% of Antarctic strains, both types of activity were detected.