Paleogenetic Analysis of the Residents from the Moscow Kremlin’s Podol (Lower Area) of the 14th Century (According to the Materials of Excavations in the Taynitsky Garden)статья
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Аннотация:Abstract. Introduction. The article presents the results of paleogenetic studies of medieval human remains ofthree people found in a closed archaeological complex (building 32) revealed during the excavations in 2007 in theTaynitsky Garden of the Moscow Kremlin (supervisor of excavations: N.A. Makarov). Previous studies on the datingof the complex links it to the devastation of Moscow by the troops of Tokhtamysh Khan in August 1382. Thearchaeological layer was formed at a time as a result of a fire and contained the remains of two adults and a 3-4 year oldchild who remained unburied. The aim of this work was the genetic study of the ancient DNA of the remains of peoplewho died in the 14th century, clarification of their gender, determination of kinship and presumptive origin. Materialand methods. For genetic examination, teeth were selected (permanent for adults, primary for a child). The laboratoryresearch algorithm included a set of measures to protect archaeological DNA from contamination, sample preparationand extraction of DNA from dental remains, analysis of STR markers of the Y chromosome in males, analysis of ALUmarkers of autosomal chromosomes, targeted NGS sequencing of hyper-variable segments of mitochondrial DNA.Results and conclusion. Using the methods of molecular genetic research, it was possible to confirm that a man, ayoung woman and a child (boy) died in the fire. Based on the analysis of autosomal markers, with a high degree ofprobability (99.9%), a close biological relationship between a woman and a child (mother-son) was revealed. The manwas not a relative of either the woman or the child. The mtDNA haplogroups and STR markers of the male specificY chromosome identified in all three individuals are generally characteristic of the Slavic population of modernEurope. The mt haplogroup J1c, found in mother and child, is now most characteristic of the inhabitants of Europe.The man has a mitochondrial haplogroup K2, which is found mainly in Northwestern Europe.