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Neuroprotective Potential of Peptides HFRWPGP (ACTH6–9PGP), KKRRPGP, and PyrRP in Cultured Cortical Neurons at Glutamate Excitotoxicityстатья
Статья опубликована в журнале из списка RSCI Web of Science
- Авторы: Bakaeva Z.V., Surin A.M., Lizunova N.V., Zgodova A.E., Krasilnikova I.A., Fisenko A.P., Frolov D.A., Andreeva L.A., Myasoedov N.F., Pinelis V.G.
- Журнал: Доклады Российской академии наук. Науки о жизни
- Том: 491
- Номер: 1
- Год издания: 2020
- Издательство: Российская академия наук
- Местоположение издательства: Москва
- Первая страница: 62
- Последняя страница: 66
- DOI: 10.1134/s1607672920020040
- Аннотация: Glutamate (Glu) excitotoxicity, which accompanies brain ischemia or traumatic brain injury, is the leading mechanism of neuronal death. In the present work, we studied the effects of the peptides HFRWPGP (ACTH6-9PGP), KKRRPG, and PyrRP on the survival of cultured cortical neurons on the background of excitotoxic effect of Glu (100 µM). Biochemical (MTT/WST) and morphometric analyzes showed that, depending on the dose, ACTH6-9PGP and KKRRPGP protect neurons from the cells death, while PyrRP, conversely, enhances it. The neuroprotective effect of ACTH6-9PGP is accompanied by a slowdown in the development of delayed calcium dysregulation and synchronous mitochondrial depolarization. Among the studied peptides, only ACTH6-9PGP significantly increased the number of neurons that restored Ca2+ homeostasis after Glu was abolished. The influence of KKRRPGP was less pronounced, whereas PyrRP, on the contrary, reduced the number of neurons with low [Ca2+]i. Thus, this study revealed the high therapeutic significance of ACTH6-9PGP and allowed assessing the prospects for its possible clinical use.
- Добавил в систему: Макарова Светлана Геннадьевна