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Effects of xenon on proinflammatory activation and apoptosis of human neutrophils (ex vivo study)тезисы доклада
- Авторы: Cherpakov R.A., Ershov A.V., Grebenchikov O.A., Antonova V.V., Kuzovlev A.N.
- Сборник: 40th International Symposium on Intensive Care & Emergency Medicine 2021
- Серия: S1
- Том: 25
- Тезисы
- Год издания: 2021
- Место издания: Springer Science and Business Media LLC
- Первая страница: 1
- Последняя страница: 2
- Номер статьи: P002
- DOI: 10.1186/s13054-021-03769-1
- Аннотация: Introduction: The aim of the study was to evaluate the effect of xenon on the activation of human neutrophils in ex vivo conditions. Methods: After receiving informed consent, 10 healthy volunteers had their blood drawn twice: before xenon inhalation (xenon - 30 vol.%, oxygen - no more than 40 vol.%, the rest - nitrogen) and immedi- ately after. The duration of inhalation in all patients was 60 min. After neutrophil isolation from the patient’s serum, lipopolysaccharide (LPS) was added to the 4 million/ml cell concentrate at a dose of 200 ng/ml. The effect of xenon on the severity of inflammatory activation of neu- trophils was assessed by the level of expression of CD11b and CD66b adhesion molecules on their surface and phosphorylation of pro- inflammatory kinases: ERK1/2 and kinase-p38. Results: The addition of lipopolysaccharide to the neutrophil incu- bation medium caused their activation, significantly increasing the phosphorylation of the key pro-inflammatory kinases of neutrophils: ERK1/2 and kinase-p38. After xenon anesthesia, there was a decrease in the expression of CD11b and CD66b adhesion molecules on the neutrophil surface and reducing the phosphorylation (activation) of pro-inflammatory kinases: ERK1/2 and MAP-kinase p38, which dem- onstrated its anti-inflammatory effect. The addition of LPS to the neutrophil incubation medium reduced their ability to spontaneous apoptosis 22 h after isolation, which was 22.6%, which was 60% less than in the control group-56.3% (p < 0.05). Xenon inhalation signifi- cantly increased to 41.35% (p < 0.05) the ability of neutrophils to spon- taneous apoptosis after incubation with LPS. Conclusions: Inhalation of xenon 30 vol% for 60 min has a pro- nounced anti-inflammatory effect on neutrophils, reducing their acti- vation by inhibiting pro-inflammatory kinases: ERK1/2 and MAP-kinase p38, reducing the expression of activation markers CD11b and CD66b on the surface of neutrophils and increasing their ability to spontane- ous apoptosis.
- Добавил в систему: Черпаков Ростислав Александрович