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Nicotiana benthamiana γ-Thionin Synthesis Is Induced in Response to Foreign Nucleus-Targeted Proteinsстатья
- Авторы: Sheshukova E., Ershova N., Lipskerov F., Komarova T.
- Журнал: Biology and Life Sciences Forum
- Том: 11
- Номер: 1
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- DOI: 10.3390/IECPS2021-12006
- Аннотация: Pathogenic and symbiotic bacteria secret protein factors—nucleomodulins—to affect thehost cell nucleus. During evolution, plants acquired a great variety of defense mechanisms including the synthesis of such antimicrobial peptides (AMPs) as defensins. We have demonstrated thattransient production of a foreign protein containing nuclear localization signal (NLS) in Nicotiana benthamiana leaves leads to the increased expression of the γ-thionin (NbγThio) that belongs to thedefensin group of AMPs. We hypothesized that NbγThio is induced by nucleomodulins of pathogenic bacteria and in particular in response to their NLSs. We used artificial nuclear proteins basedon green fluorescent protein (GFP) fused with the human prothymosin α NLS or VirE3 NLS fromAgrobacterium tumefaciens as mimetics of bacterial effectors. We demonstrated that superproductionof these NLS-containing reporters in the transient expression system in N. benthamiana leaves resulted in the increase of NbγThio mRNA level. We isolated the NbγThio gene promoter (PrγThio) andcreated an expression vector (PrγThio-GUS) directing GUS synthesis in agroinfiltrated leaves. Co-expression of PrγThio-GUS with 35S-GFP:NLS variants led to the significant stimulation of GUS synthesis. We concluded that NbγThio gene expression is activated in response to bacterial nucleus-targeted proteins in the cell via induction of PrγThio.
- Добавил в систему: Комарова Татьяна Валерьевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Sheshukova_BLSF_2022.pdf | 948,9 КБ | 30 марта 2022 [tvkomarova] |