Na+ and K+ Ions Differently Affect Nucleosome Structure, Stability, and Interactions with Proteins

Andreeva, T.V.; Maluchenko, N.V.; Sivkina, A.L.; Chertkov, O.V.; Valieva, M.E.; Kotova, E.Y.; Kirpichnikov, M.P.; Studitsky, V.M.; Feofanov, A.V.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 апреля 2022 г.

Авторы: Andreeva Tatyana V., Maluchenko Natalya V., Sivkina Anastasiia L., Chertkov Oleg V., Valieva Maria E., Kotova Elena Y., Kirpichnikov Mikhail P., Studitsky Vasily M., Feofanov Alexey V.
Журнал: Microscopy and Microanalysis
Том: 28
Номер: 1
Год издания: 2022
Издательство: Cambridge University Press
Местоположение издательства: United Kingdom
Первая страница: 243
Последняя страница: 253
DOI: 10.1017/s1431927621013751
Аннотация: Inorganic ions are essential factors stabilizing nucleosome structure; however, many aspects of their effects on DNA transactions in chromatin remain unknown. Here, differential effects of K+ and Na+ on the nucleosome structure, stability, and interactions with protein complex FACT (FAcilitates Chromatin Transcription), poly(ADP-ribose) polymerase 1, and RNA polymerase II were studied using primarily single-particle Förster resonance energy transfer microscopy. The maximal stabilizing effect of K+ on a nucleosome structure was observed at ca. 80–150 mM, and it decreased slightly at 40 mM and considerably at >300 mM. The stabilizing effect of Na+ is noticeably lower than that of K+ and progressively decreases at ion concentrations higher than 40 mM. At 150 mM, Na+ ions support more efficient reorganization of nucleosome structure by poly(ADP-ribose) polymerase 1 and ATP-independent uncoiling of nucleosomal DNA by FACT as compared with K+ ions. In contrast, transcription through a nucleosome is nearly insensitive to K+ or Na+ environment. Taken together, the data indicate that K+ environment is more preserving for chromatin structure during various nucleosome transactions than Na+ environment.
Добавил в систему: Сивкина Анастасия Львовна

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

Na+ and K+ Ions Differently Affect Nucleosome Structure, Stability, and Interactions with Proteinsстатья

Прикрепленные файлы