Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17beta-hydroxysteroid dehydrogenase activities

Kollerov, V.; Shutov, A.; Kazantsev, A.; Donova, M.

Авторы: Kollerov Vyacheslav, Shutov Andrei, Kazantsev Alexey, Donova Marina
Журнал: Fungal Biology
Том: 126
Номер: 1
Год издания: 2022
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 91
Последняя страница: 100
DOI: 10.1016/j.funbio.2021.11.002
Аннотация: Fungal strain Drechslera sp. Ph F-34 was shown to modify 3-oxo- and 3-hydroxy steroids of androstane series to form the corresponding allylic 7-alcohols and 17β-reduced derivatives thus evidencing the presence of 7α-, 7β-hydroxylase and 17β-hydroxysteroid dehydrogenase (17β-HSD) activities. The growing mycelium predominantly hydroxylated androsta-1,4-diene-3,17-dione (ADD) at the 7β-position, while much lower 7α-hydroxylation was observed. Along with 7β-hydroxy-ADD and its corresponding 7α-isomer, their respective 17β-alcohols were produced.In this study, transformation of ADD, androst-4-en-17β-ol-3-one (testosterone, TS) and 3β-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA) by resting mycelium of Drechslera sp. have been estimated in different conditions with regard to the inducibility and functionality of the 17β-HSD and 7-hydroxylase enzyme systems. Steroids of androstane, pregnane and cholane series were evaluated as inducers. The inhibitory analysis was provided using cycloheximide (CHX). Steroids were assayed using TLC and HPLC methods, and the structures were confirmed by mass-spectrometry, 1H and 13C NMR spectroscopy data.
Добавил в систему: Казанцев Алексей Витальевич

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Steroid modification by filamentous fungus Drechslera sp.: Focus on 7-hydroxylase and 17beta-hydroxysteroid dehydrogenase activitiesстатья Исследовательская статья

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