Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

Gorbunov, A.A.; Akentyev, F.I.; Gubaidullin, I.I.; Zhiganov, N.I.; Tereshchenkova, V.F.; Elpidina, E.N.; Kozlov, D.G.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 января 2022 г.

Авторы: Gorbunov A.A., Akentyev F.I., Gubaidullin I.I., Zhiganov N.I., Tereshchenkova V.F., Elpidina E.N., Kozlov D.G.
Журнал: Applied Biochemistry and Microbiology
Том: 57
Номер: 9
Год издания: 2021
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 917
Последняя страница: 924
DOI: 10.1134/s0003683821090039
Аннотация: A strain of the yeast Komagataella kurtzmanii, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of proenzyme secretion was 20–50 mg/L. It was shown that the target His6-tagged protein was produced in two forms during secretion in yeast. One of them was a monomer that was efficiently purified via Ni-NTA chromatography and then activated with trypsin. Another form accumulated in the culture medium as oligomers prone to aggregation in the presence of Ni2+ ions and was not activated by trypsin treatment. Aggregation is likely the result of the polypeptide-chain misfolding.
Добавил в систему: Элпидина Елена Николаевна

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИПМех РАН

Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmaniiстатья

Прикрепленные файлы