Diversity and Activity of Sulfate-Reducing Prokaryotes in Kamchatka Hot Springsстатья
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Дата последнего поиска статьи во внешних источниках: 22 декабря 2021 г.
Аннотация:Microbial communities of the Kamchatka Peninsula terrestrial hot springs were studiedusing radioisotopic and cultural approaches, as well as by the amplification and sequencing ofdsrB and 16S rRNA genes fragments. Radioisotopic experiments with 35S-labeled sulfate showedthat microbial communities of the Kamchatka hot springs are actively reducing sulfate. Both thecultivation experiments and the results of dsrB and 16S rRNA genes fragments analyses indicatedthe presence of microorganisms participating in the reductive part of the sulfur cycle. It was foundthat sulfate-reducing prokaryotes (SRP) belonging to Desulfobacterota, Nitrospirota and Firmicutesphyla inhabited neutral and slightly acidic hot springs, while bacteria of phylum Thermodesulofobiotapreferred moderately acidic hot springs. In high-temperature acidic springs sulfate reduction wasmediated by archaea of the phylum Crenarchaeota, chemoorganoheterotrophic representatives ofgenus Vulcanisaeta being the most probable candidates. The 16S rRNA taxonomic profiling showedthat in most of the studied communities SRP was present only as a minor component. Only in onemicrobial community, the representatives of genus Vulcanisaeta comprised a significant group. Thus,in spite of comparatively low sulfate concentrations in terrestrial hot springs of the Kamchatka,phylogenetically and metabolically diverse groups of sulfate-reducing prokaryotes are operatingthere coupling carbon and sulfur cycles in these habitats.