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Calcium Imaging Reveals Fast Tuning Dynamics of Hippocampal Place Cells and CA1 Population Activity during Free Exploration Task in Miceстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Sotskov V.P., Pospelov N.A., Plusnin V.V., Anokhin K.V.
- Журнал: International Journal of Molecular Sciences
- Номер: 23, 638
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Аннотация: DOI: https://doi.org/10.3390/ijms23020638Abstract: Hippocampal place cells are a well-known object in neuroscience, but their place fieldformation in the first moments of navigating in a novel environment remains an ill-defined process.To address these dynamics, we performed in vivo imaging of neuronal activity in the CA1 field ofthe mouse hippocampus using genetically encoded green calcium indicators, including the novelNCaMP7 and FGCaMP7, designed specifically for in vivo calcium imaging. Mice were injected witha viral vector encoding calcium sensor, head-mounted with an NVista HD miniscope, and allowedto explore a completely novel environment (circular track surrounded by visual cues) without any reinforcement stimuli, in order to avoid potential interference from reward-related behavior. First, we calculated the average time required for each CA1 cell to acquire its place field. We found that 25% of CA1 place fields were formed at the first arrival in the corresponding place, while the average tuning latency for all place fields in a novel environment equaled 247 s. After 24 h, when the environment was familiar to the animals, place fields formed faster, independent of retention of cognitive mapsduring this session. No cumulation of selectivity score was observed between these two sessions.Using dimensionality reduction, we demonstrated that the population activity of rapidly tuned CA1place cells allowed the reconstruction of the geometry of the navigated circular maze; the distribution of reconstruction error between the mice was consistent with the distribution of the average place field selectivity score in them. Our data thus show that neuronal activity recorded with genetically encoded calcium sensors revealed fast behavior-dependent plasticity in the mouse hippocampus, resulting in the rapid formation of place fields and population activity that allowed the reconstruction of the geometry of the navigated maze.
- Добавил в систему: Анохин Константин Владимирович