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Transcriptome sequencing revealed differences in the response of renal cancer cells to hypoxia and CoCl2 treatmentстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 апреля 2022 г.
- Авторы: Zhigalova Nadezhda, Artemov Artem, Mazur Alexander M., Prokhortchouk Egor B.
- Журнал: F1000Research
- Том: 4
- Год издания: 2015
- Первая страница: 1518
- DOI: 10.12688/f1000research.7571.1
- Аннотация: Human cancer cells are subjected to hypoxic conditions in many tumours. Hypoxia causes alterations in the glycolytic pathway activation through stabilization of hypoxia-inducible factor 1. Currently, two approaches are commonly used to model hypoxia: an alternative to generating low-oxygen conditions in an incubator, cells can be treated with CoCl2. We performed RNA-seq experiments to study transcriptomes of human Caki-1 cells under real hypoxia and after CoCl2 treatment. Despite causing transcriptional changes of a much higher order of magnitude for the genes in the hypoxia regulation pathway, CoCl2 treatment fails to induce alterations in the glycolysis / gluconeogenesis pathway. Moreover, CoCl2 caused aberrant activation of other oxidoreductases in glycine, serine and threonine metabolism pathways.
- Добавил в систему: Артемов Артем Владимирович