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Design of sequence-specific DNA-binding ligands that use two-stranded peptide motif for DNA sequence recognitionстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 января 2017 г.
- Авторы: Nikolaev V.A., Grokhovsky S.L., Zhuze A.L., Surovaya A.N., Leinsoo T.A., Sidorova N.Yu, Zasedatelev A.S., Strahan G.A., Shafer R.H., Gursky G.V.
- Журнал: Journal of Biomolecular Structure and Dynamics
- Том: 14
- Год издания: 1996
- Издательство: Adenine Press
- Местоположение издательства: United States
- Первая страница: 31
- Последняя страница: 47
- Аннотация: The design and DNA binding activity of beta-structure-forming peptides and netropsin-peptide conjugates are reported. It is found that a pair of peptides-S,S'-bis(Lys-Gly-Val-Cys-Val-NH-NH-Dns)-bridged by an S-S bond binds at least 10 times more strongly to poly(dG).poly(dC) than to poly(dA).poly(dT). This peptide can also discriminate between 5'-GpG-3' and 5'-GpC-3' steps in the DNA minor groove. Based on these observations, new synthetic ligands, bis-netropsins, were constructed in which two netropsin-like fragments were attached by means of short linkers to a pair of peptides-Gly-Cys-Gly- or Val-Cys-Val-bridged by S-S bonds. These compounds possess a composite binding specificity: the peptide chains recognize 5'-GpG-3' steps on DNA, whereas the netropsin-like fragments bind preferentially to runs of 4 AT base pairs. Our data indicate that combining the AT-base-pair specific properties of the netropsin-type structure with the 5'-GpG-3'-specific properties of certain oligopeptides offers a new approach to the synthesis of ligands capable of recognizing mixed sequences of AT- and GC-base pairs in the DNA minor groove. These compounds are potential models for DNA-binding domains in proteins which specifically recognize base pair sequences in the minor groove of DNA.
- Добавил в систему: Гурский Георгий Валерианович