Application of Prussian Blue modified carbon electrodes for amperometric detection of amyloid-β peptides by flow injection analysisстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 8 апреля 2022 г.
Аннотация:Prussian Blue (PB) modified carbon screen printed electrodes (SPE) were successfully applied for detection of amyloid-β (Aβ) peptides of various lengths and amino acid sequences by amperometric flow injection analysis (FIA). A pronounced catalytic effect of PB (10- to 27-fold current increase) was found for peptides lacking the Tyr-10 residue, viz. Aβ(1–7)-D7H, rat Aβ(1–16), and Aβ(1–16)-Y10A, while practically no effect of PB was observed for Aβ(1–16)-D7H, Aβ(1–16)-H6R, and Aβ(1–16)-E11A mutants. Only a 1.5-fold current enhancement was observed for Aβ(1–16) oxidation on SPE/PB, compared to SPE, by FIA. Therefore, the combination of FIA and SPE/PB may open a new avenue for the electrochemical investigation of peptides of interest lacking inherently electroactive residues (Tyr, Trp, Cys, His, and Met), as exemplified by naturally occurring and artificial Aβ mutants containing no Tyr-10 residue.