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A lichenase-like family 12 endo-(l -> 4)-beta-glucanase from Aspergillus japonicus: study of the substrate specificity and mode of action on beta-glucans in comparison with other glycoside hydrolasesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Grishutin S.G., Gusakov A.V., Dzedzyulya E.I., Sinitsyn A.P.
- Журнал: Carbohydrate Research
- Том: 341
- Номер: 2
- Год издания: 2006
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 218
- Последняя страница: 229
- DOI: 10.1016/j.carres.2005.11.011
- Аннотация: Using anion-exchange chromatography on Source 15Q followed by hydrophobic interaction chromatography on Source 15 Isopropyl, a lichenase-like endo-(1 -> 4)-beta-glucanase (BG, 28 kDa, pI 4.1) was isolated from a culture filtrate of Aspergillus japonicus. The enzyme was highly active against barley beta-glucan and lichenan (263 and 267 U/mg protein) and had much lower activity toward carboxymethylcellulose (3.9 U/mg). The mode of action of the BG on barley beta-glucan and lichenan was studied in comparison with that of Bacillus subtilis lichenase and endo-(1 -> 4)-beta-glucanases (EG 1, 11, and 111) of Trichoderma reesei. The BG behaved very similar to the bacterial lichenase, except the tri- and tetrasaccharides formed as the end products of beta-glucan hydrolysis with the BG contained the beta-(1 -> 3)-glucoside linkage at the non-reducing end, while the lichenase-derived oligosaccharides had the beta-(1 -> 3)linkage at the reducing end. The BG was characterized by a high amino acid sequence identity to the EG of Aspergillus kawachii (UniProt entry Q12679) from a family 12 of glycoside hydrolases (96% in 162 identified aa residues out of total 223 residues) and also showed lower sequence similarity to the EglA of Aspergillus niger (074705). (c) 2005 Elsevier Ltd. All rights reserved.
- Добавил в систему: Гусаков Александр Васильевич