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CD95-deficient cells of Jurkat/A4 subline are resistant to drug-induced apoptosisстатья
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.
- Авторы: Sokolovskaya A.A., Zabotina T.N., Blokhin D.Yu, Inshakov A.N., Mikhailov A.D., Kadagidze Z.G., Baryshnikov A.Yu
- Журнал: Experimental oncology
- Том: 23
- Номер: 3
- Год издания: 2001
- Издательство: Morion
- Местоположение издательства: Kyiv, Ukraine
- Первая страница: 175
- Последняя страница: 180
- Аннотация: We developed unique CD95-deficient subline (Jurkat/A4) from the parent Jurkat/WT cell line by continuous culture in medium containing apoptosis-inducing anti-CD95 MAb, clone IPO-4 (IgM). In contrast to the parental Jurkat/WT line, which displays relatively high expression of CD95 (60-80% antigene-positive cells), cell-surface expression of the CD95 was entirely negative in the Jurkat/A4 cell line (only 4% of antigene-positive cells). The Jurkat/A4 cells are resistant to induction of apoptosis by both anti-CD95 mAb IPO-4 and chemotherapeutic drugs, whereas the wild type clone Jurkat/WT undergoes time-dependent apoptosis. Thus, these results suggest the possible participation of CD95 pathway in the development of drug-induced apoptosis.
- Добавил в систему: Заботина Татьяна Николаевна