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Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activitiesстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Gusakov Alexander V., Kondratyeva Elena G., Sinitsyn Arkady P.
- Журнал: International journal of analytical chemistry
- Том: 2011
- Год издания: 2011
- Издательство: Hindawi Pub. Corp.
- Местоположение издательства: New York, United States
- Первая страница: 283658
- DOI: 10.1155/2011/283658
- Аннотация: The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS assays in determination of cellulase, beta-glucanase, xylanase, and beta-mannanase activities was carried out. When cellulase activities against CMC were measured, the DNS assay gave activity values, which were typically 40-50% higher than those obtained with the NS assay. In the analysis of the xylanase, beta-mannanase, and beta-glucanase activities, the overestimations by the DNS assay were much more pronounced (the observed differences in the activities were 3- to 13-fold). Reasons for preferential use of the NS assay for measuring activities of carbohydrases other than cellulases are discussed.
- Добавил в систему: Гусаков Александр Васильевич