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Hydroxylation of pregnenolone and dehydroepiandrosterone by zygomycete Backusella lamprospora VKM F-944: selective production of 7-alpha-OH-DHEAстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2022 г.
- Авторы: Kollerov Vyacheslav, Shutov Andrei, Kazantsev Alexey, Donova Marina
- Журнал: Applied Microbiology and Biotechnology
- Том: 106
- Номер: 2
- Год издания: 2022
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 535
- Последняя страница: 548
- DOI: 10.1007/s00253-021-11737-6
- Аннотация: In this paper, we studied the transformation of two 3β-hydroxy-5-ene-steroids—pregnenolone and dehydroepiandrosterone (DHEA) by Backusella lamprospora VKM F-944. The soil-dwelling zygomycete wild-type strain has been earlier selected during the screening and previously unexplored for this purpose. The fungus fully converted pregnenolone to form a mixture of axial 7α-hydroxy-pregnenolone and 7α,11α-dihydroxy-pregnenolone, while no metabolites with β-orientation of the hydroxyl group were detected. The pathway to 7α,11α-diOH-pregnenolone seems to include 7α-hydroxylation of 11α-hydroxylated derivative. The only product from DHEA was identified as 7α-hydroxy-DHEA. The structures of steroid metabolites were confirmed by HPLC, mass-spectrometry (MS), and 1H and 13C NMR analyses. Under the optimized conditions, the yield of 7α-OH-DHEA reached 94% (w/w) or over 14 g/L in absolute terms, even at high concentration of the substrate (DHEA) (15 g/L). To our knowledge, it is the highest yield of the value-added 7α-OH-DHEA reported so far.
- Добавил в систему: Казанцев Алексей Витальевич