|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Fluorescence polarisation immunoassays for strobilurin fungicides kresoxim-methyl, trifloxystrobin and picoxystrobinстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 февраля 2017 г.
- Авторы: Kolosova Anna, Maximova Ksenia, Eremin Sergei A., Zherdev Anatoly V., Mercader Josep V., Abad-Fuentes Antonio, Dzantiev Boris B.
- Журнал: Talanta
- Том: 162
- Год издания: 2017
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 495
- Последняя страница: 504
- DOI: 10.1016/j.talanta.2016.10.063
- Аннотация: Fluorescence polarisation immunoassays (FPIAs) based on monoclonal antibodies for detection of three strobilurin fungicides - kresoxim-methyl (KM), trifloxystrobin (TF) and picoxystrobin (PC), were developed and optimised. Fluorescein-labeled derivatives of target antigens (tracers) were synthesised and purified by thin-layer chromatography. Influence of tracer structures on the assay parameters was investigated. For KM and TF, the best assay performance was achieved with the homologous pairs of reagents. For the PC assay, the heterologous tracer, i.e. fluorescein-labeled derivative of TF, was used. The developed FPIAs were applied to the determination of KM, TF and PC in red wine. Most optimal sample preparation was achieved with cross-linked poly(vinylpyrrolidone) as a sorbent. This clean-up is simple, rapid and allows determination of all three strobilurin fungicides in one sample. Detection limits of the developed FPIAs in red wine were 28, 6 and 5 ng/mL for KM, TF and PC, respectively. Recovery in spiked samples averaged between 80 and 104%. The developed FPIA methods can be applied to screening of wine samples for KM, TF and PC residues without complicated cleanup.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 17_Talanta162_495_FPIA_strobin.pdf | 17_Talanta162_495_FPIA_strobin.pdf | 784,6 КБ | 28 января 2017 [Sergei_A_EREMIN] |