Glutamine effect on cultured granule neuron death induced by glucose deprivation and chemical hypoxiaстатья
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 7 октября 2013 г.
Местоположение издательства:Road Town, United Kingdom
Первая страница:1039
Последняя страница:1044
Аннотация:Using a specific fluorescent probe of mitochondrial membrane potential
(tetramethylrhodamine ethyl ester), we have shown that glucose deprivation (GD)
of cultured cerebellar granule neurons (CGN) for 3 h lowers mitochondrial
membrane potential in these cells. Longer glucose starvation (24 h) causes CGN
death that is not prevented by blockers of ionotropic glutamate receptors (MK-801
(10 µM) and NBQX (10 µM)). Glutamine or pyruvate (2 mM) maintain membrane
potential of mitochondria and decrease CGN death under GD conditions. In the
presence of glucose the mitochondrial respiratory chain blocker rotenone induces
neuron death potentiated by glutamine. The potentiation effect is completely
prevented by blockers of ionotropic glutamate receptors. These results show that
glutamine under conditions of GD can be utilized by mitochondria as substrate,
but at the same time, in the case of mitochondrial function deterioration,
metabolism of this amino acid results in glutamate accumulation to toxic level.