|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modificationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Trashin Stanislav A., Haltrich Dietmar, Ludwig Roland, Gorton Lo, Karyakin Arkady A.
- Журнал: Bioelectrochemistry
- Том: 76
- Номер: 1-2
- Год издания: 2009
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 87
- Последняя страница: 92
- DOI: 10.1016/j.bioelechem.2009.06.004
- Аннотация: Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron transfer with that obtained through mediated electron transfer. it was clearly observed that the improved current of the SPE vertical bar PANI vertical bar CDH electrode is due to the specific role of PANI, rather than caused by a rise of enzyme loading. The operational stability of the enzyme electrode based on PANI modified SPE was 5 times higher compared with that based on plain SPE. (C) 2009 Elsevier B.V. All rights reserved
- Добавил в систему: Карякин Аркадий Аркадьевич