Fungal Enzyme l-Lysine alpha-Oxidase Affects the Amino Acid Metabolism in the Brain and Decreases the Polyamine Level

Lukasheva, E.; Makletsova, M.; Lukashev, A.; Babayeva, G.; Arinbasarova, A.; Medentsev, A.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 1 апреля 2022 г.

Авторы: Lukasheva Elena, Makletsova Marina, Lukashev Alexander, Babayeva Gulalek, Arinbasarova Anna, Medentsev Alexander
Журнал: Pharmaceuticals
Том: 13
Номер: 11
Год издания: 2020
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 398
DOI: 10.3390/ph13110398
Аннотация: The fungal glycoprotein l-lysine α-oxidase (LO) catalyzes the oxidative deamination of l-lysine (l-lys). LO may be internalized in the intestine and shows antitumor, antibacterial, and antiviral effects in vivo. The main mechanisms of its effects have been shown to be depletion of the essential amino acid l-lys and action of reactive oxidative species produced by the reaction. Here, we report that LO penetrates into the brain and is retained there for up to 48 h after intravenous injection, which might be explained by specific pharmacokinetics. LO actively intervenes in amino acid metabolism in the brain. The most significant impact of LO was towards amino acids, which are directly exposed to its action (l-lys, l-orn, l-arg). In addition, the enzyme significantly affected the redistribution of amino acids directly associated with the tricarboxylic acid (TCA) cycle (l-asp and l-glu). We discovered that the depletion of l-orn, the precursor of polyamines (PA), led to a significant and long-term decrease in the concentration of polyamines, which are responsible for regulation of many processes including cell proliferation. Thus, LO may be used to reduce levels of l-lys and PA in the brain.
Добавил в систему: Бабаева Гулалек

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Fungal Enzyme l-Lysine alpha-Oxidase Affects the Amino Acid Metabolism in the Brain and Decreases the Polyamine Levelстатья Исследовательская статья

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