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Enhanced chemiluminescence: A sensitive analytical system for detection of sweet potato peroxidaseстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Vdovenko Marina M., Della Ciana Leopoldo, Sakharov Ivan Y.
- Журнал: Biotechnology journal
- Том: 5
- Номер: 8
- Год издания: 2010
- Издательство: Wiley - VCH Verlag GmbH & CO. KGaA
- Местоположение издательства: Germany
- Первая страница: 886
- Последняя страница: 890
- DOI: 10.1002/biot.201000033
- Аннотация: 3-(10'-Phenothiazinyl)propane-l-sulfonate (SPTZ) was shown to be a potent enhancer of anionic sweet potato peroxidase (aSPP)-induced chemiluminescence The optimal conditions for aSPP-catalyzed oxidation of luminol were investigated by varying the concentrations of luminol, hydrogen peroxide, Tris, and SPTZ as well as the pH values of the reaction mixture Addition of 4-morphohnopyridine (MORP) to the reaction mixture markedly increased the light intensity Using SPTZ and MORP together enhanced the effect 265 times The lower detection limit (LDL) of SPP was 009 pM, approximately in 10 times lower than that for the cationic isozyme c of horseradish peroxidase/4-iodophenol system It was shown that aSPP in the presence of SPTZ produced a longer lasting chemiluminescent signal
- Добавил в систему: Сахаров Иван Юрьевич