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Disaccharide synthesis by enzymatic condensation of glucose: glycoside linkage patterns for different fungal speciesстатья
- Авторы: Semenova M.V., Okunev O.N., Gusakov A.V., Sinitsyn A.P.
- Журнал: Open glycoscience
- Том: 2
- Год издания: 2009
- Издательство: Bentham Science Publishers
- Местоположение издательства: Netherlands
- Первая страница: 20
- Последняя страница: 24
- DOI: 10.2174/1875398100902010020
- Аннотация: Four enzyme preparations produced by fungal species belonging to different genera (Aspergillus niger, Corynascus sp., Penicillium verruculosum, Trichoderma reesei) were used for synthesis of disaccharides by D-glucose (60% w/v) condensation catalyzed by beta-glucosidase. Effects of pH and temperature on the disaccharide synthesis were studied, and glycoside linkage patterns for enzymes from different sources were determined. The highest concentration of disaccharides (114 and 118 g/l) was achieved in the case of A. niger and Corynascus sp. enzymes after 48 h of the condensation reaction carried out at 70oC and optimal pH; the P. verruculosum sample slightly conceded them in the yield of products (96 g/l), while the T. reesei preparation displayed the lowest synthetic activity (35 g/l). Gentiobiose was predominantly formed in the reaction catalyzed by the first three enzyme samples, while in the case of T. reesei laminaribiose was the main condensation product.
- Добавил в систему: Гусаков Александр Васильевич