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Induction of ischemic tolerance in rat cortical neurons by 3-nitropropionic acid: chemical preconditioningстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Weih M., Bergk A., Isaev NK, Ruscher K., Megow D., Riepe M., Meisel A., Victorov IV, Dirnagl U.
- Журнал: Neuroscience Letters
- Том: 272
- Номер: 3
- Год издания: 1999
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 207
- Последняя страница: 210
- Аннотация: Sublethal ischemia leads to increased tolerance against subsequent ischemia. We investigated whether tolerance could also be elicited by mild respiratory-chain inhibition (chemical hypoxia) in a rat neuronal-cell enriched culture system. 3-Nitropropionic acid (3-NPA) caused a concentration-dependent inhibition of succinate-dehydrogenase. Two hours preconditioning with 3-NPA 24-48 h before oxygen-glucose deprivation (OGD) reduced neuronal damage morphologically and reduced lactate deydrogenase (LDH) release up to 72% compared to sham-treated sister cultures without 3-NPA. In an attempt to elucidate transcriptional mechanisms, we found no rapid translocation of the hypoxia-sensitive transcription factors N F-KB or hypoxia-inducible factor-I (HIF-I) at 3-NPA concentrations sufficient to trigger tolerance against OGD. In accordance to previous in vivo and brain slice data, we conclude that 3-NPA chemically induces tolerance against oxygen-glucose deprivation in vitro. However, the underlying mechanisms remain elusive.
- Добавил в систему: Исаев Николай Константинович