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DETERMINATION OF THE MAXIMUM LENGTH OF DNA IN A POLYMER BASED ON LINEAR POLY(N,N-DIMETHYLACRYLAMIDE) DECODED WITH AN ACCURACY OF 99% BY CAPILLARY GEL ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCEстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 апреля 2022 г.
- Авторы: Bocharova D.V., Alekseev Y.I., Lavrov G.S., Bardin B.V., Kurochkin V.E., Plugov A.G., Volkov I.A., Chemigov A.A., Volkov A.A.
- Журнал: Journal of Analytical Chemistry
- Том: 76
- Номер: 12
- Год издания: 2021
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 1408
- Последняя страница: 1413
- DOI: 10.1134/S106193482110004X
- Аннотация: DNA sequencing is an essential task of modern molecular genetic research. Its fundamentalparameter is the accuracy of decoding. In this work, we determined the maximum lengths of DNA fragments,obtained by the Sanger method and sequenced with an accuracy of 99%, in a polymer based on linearpoly(N,N-dimethylacrylamide) PDMA-6 for capillaries 50 cm long to the optical window of the detector ofa Nanophore 05 domestic genetic analyzer and an imported GA 3500xL genetic analyzer. The results of DNAsequencing obtained by various analysis algorithms were compared. The parameters of the analysis algo-rithms were selected, ensuring best results of DNA sequencing in using the PDMA-6 polymer.
- Добавил в систему: Бочарова Дария Владимировна