Genes Responsiblefor H2S Production and MetabolismAre Involved in Learning andMemory in Drosophila melanogaster

Zatsepina, O.G.; Chuvakova, L.N.; Nikitina, E.A.; Rezvykh, A.P.; Zakluta, A.S.; Sarantseva, S.V.; Surina, N.V.; Ksenofontov, A.L.; Baratova, L.A.; Shilova, V.Y.; Michael, B.E.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 13 июля 2022 г.

Авторы: Zatsepina O.G., Chuvakova L.N., Nikitina E.A., Rezvykh A.P., Zakluta A.S., Sarantseva S.V., Surina N.V., Ksenofontov A.L., Baratova L.A., Shilova V.Y., Michael B.Evgen’ev
Журнал: Biomolecules
Том: 12
Номер: 6
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 751
DOI: 10.3390/biom12060751
Аннотация: The gasotransmitter hydrogen sulfide (H2S) produced by the transsulfuration pathway(TSP) is an important biological mediator, involved in many physiological and pathological processesin multiple higher organisms, including humans. Cystathionine--synthase (CBS) and cystathionine--lyase (CSE) enzymes play a central role in H2S production and metabolism. Here, we investigatedthe role of H2S in learning and memory processes by exploring several Drosophila melanogaster strainswith single and double deletions of CBS and CSE developed by the CRISPR/Cas9 technique. Wemonitored the learning and memory parameters of these strains using the mating rejection courtshipparadigm and demonstrated that the deletion of the CBS gene, which is expressed predominantly inthe central nervous system, and double deletions completely block short- and long-term memoryformation in fruit flies. On the other hand, the flies with CSE deletion preserve short- and long-termmemory but fail to exhibit long-term memory retention. Transcriptome profiling of the heads of themales from the strains with deletions in Gene Ontology terms revealed a strong down-regulation ofmany genes involved in learning and memory, reproductive behavior, cognition, and the oxidation–reduction process in all strains with CBS deletion, indicating an important role of the hydrogensulfide production in these vital processes.
Добавил в систему: Ксенофонтов Александр Леонидович

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Genes Responsiblefor H2S Production and MetabolismAre Involved in Learning andMemory in Drosophila melanogasterстатья Исследовательская статья

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