Assembly of photoactive orange carotenoid protein from its domains unravels a carotenoid shuttle mechanism

Moldenhauer, M.; Sluchanko, N.N.; David, B.; Zlenko, D.V.; Tavraz, N.N.; Franz‑Josef, S.; Peter, H.; Maksimov, E.G.; Thomas, F.

Авторы: Marcus Moldenhauer, Sluchanko Nikolai N., David Buhrke, Zlenko Dmitry V., Tavraz Neslihan N., Franz‑Josef Schmitt, Peter Hildebrandt, Maksimov Eugene G., Thomas Friedrich
Журнал: Photosynthesis Research
Год издания: 2017
Издательство: Springer Nature
Местоположение издательства: Switzerland
DOI: 10.1007/s11120-017-0353-3
Аннотация: Cyanobacterial genomes frequently harbor, besides genes for Orange Carotenoid Proteins (OCPs), several genes coding for homologs of OCP’s individual N- or C-terminal domains (NTD, CTD). While the role of the OCP-NTD homologs, also known as Red Carotenoid Proteins (RCPs), in cyanobacterial photoprotection is well established, the role of OCP-CTD homologs is elusive. OCP binds ketocarotenoids and, upon photoconversion from the orange OCPO to the red OCPR state, quenches the fluorescence of phycobilisome antennae, thereby preventing excessive energy flow to photosynthetic reaction centers. We show how OCP can be reconstructed from its functional domains. Upon expression of the OCP-CTD in carotenoid-producing E. coli strains, unexpected violet-colored proteins were obtained, which, upon mixing with the RCP-apoprotein, resulted in an orange-like photoswitchable species that converted upon further illumination into a species spectroscopically indistinguishable from RCP. The OCP-CTD itself is a novel, dimeric carotenoid-binding protein, which effectively quenches singlet oxygen and forms complex with the Fluorescence Recovery Protein. While formation of RCP from the dimeric OCP-CTD via a photoswitchable OCP-like intermediate demonstrates a unique carotenoid shuttle mechanism, the properties of the OCP-CTD reported here suggest physiological roles to the multitude of OCP-CTD homologs in other species and explain the evolutionary process of OCP formation.
Добавил в систему: Максимов Евгений Георгиевич

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Assembly of photoactive orange carotenoid protein from its domains unravels a carotenoid shuttle mechanismстатья

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